Anti-RAC3 抗体 [EPR6679(B)] (ab124943)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6679(B)] to RAC3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-RAC3 antibody [EPR6679(B)]
RAC3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6679(B)] to RAC3 -
由来種
Rabbit -
特異性
ab124943 cross-reacts with Rac1, Rac2 and Cdc42 -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human RAC3 aa 50-150. The exact sequence is proprietary.
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ポジティブ・コントロール
- Human heart tissue, Human placenta tissue; JAR cells; Human fetal brain lysate, SH-SY5Y, JAR and BxPC-3 cell lysates
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 1.23 x 10 -10 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6679(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124943の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
antigen retrieval is recommeded. |
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ICC/IF |
1/50 - 1/100.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 21 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. antigen retrieval is recommeded. |
ICC/IF
1/50 - 1/100. |
ターゲット情報
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機能
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as cell spreading and the formation of actin-based protusions including lamellipodia and membrane ruffles. Promotes cell adhesion and spreading on fibrinogen in a CIB1 and alpha-IIb/beta3 integrin-mediated manner. -
組織特異性
Highest levels in brain, also detected in heart, placenta and pancreas. -
配列類似性
Belongs to the small GTPase superfamily. Rho family. -
翻訳後修飾
(Microbial infection) Glycosylated at Tyr-32 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of Rac and leads to actin disassembly. -
細胞内局在
Cytoplasm. Endomembrane system. Cell projection, lamellipodium. Cytoplasm, perinuclear region. Cell membrane. Cytoplasm, cytoskeleton. Membrane-associated when activated. Colocalizes with NRBP to endomembranes and at the cell periphery in lamellipodia. Colocalized with CIB1 in the perinuclear area and at the cell periphery. - Information by UniProt
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参照データベース
- Entrez Gene: 5881 Human
- Entrez Gene: 170758 Mouse
- Omim: 602050 Human
- SwissProt: P60763 Human
- SwissProt: P60764 Mouse
- Unigene: 45002 Human
- Unigene: 34008 Mouse
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製品の状態
Rac3 has high sequence homology with other Rac proteins. Therefore some antibodies may show cross-reactivity. -
別名
- OTTMUSP00000004488 antibody
- p21 Rac3 antibody
- p21-Rac3 antibody
see all
画像
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All lanes : Anti-RAC3 antibody [EPR6679(B)] (ab124943) at 1/1000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : JAR cell lysate
Lane 4 : BxPC-3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 21 kDa -
ab124943, at a 1/50 dilution, staining RAC3 in paraffin-embedded Human heart tissue by immunohistochemistry
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab124943, at a 1/50 dilution, staining RAC3 in paraffin-embedded Human placenta tissue by immunohistochemistry
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab124943, at a 1/50 dilution, staining RAC3 in JAR cells by immunofluorescence
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab124943 は 4 報の論文で使用されています。
- Cheng C et al. RAC3 Promotes Proliferation, Migration and Invasion via PYCR1/JAK/STAT Signaling in Bladder Cancer. Front Mol Biosci 7:218 (2020). PubMed: 33062641
- Gaitanos TN et al. Tiam-Rac signaling mediates trans-endocytosis of ephrin receptor EphB2 and is important for cell repulsion. J Cell Biol 214:735-52 (2016). PubMed: 27597758
- Wang Y et al. Inhibition of prostate smooth muscle contraction and prostate stromal cell growth by the inhibitors of Rac, NSC23766 and EHT1864. Br J Pharmacol 172:2905-17 (2015). PubMed: 25631101
- Wang H et al. Overexpression of RhoGDI, a novel predictor of distant metastasis, promotes cell proliferation and migration in hepatocellular carcinoma. FEBS Lett 588:503-8 (2014). PubMed: 24374343