製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to Rabex5
  • 由来種
    Mouse
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length human Rabex5 (NP_055319).

  • ポジティブ・コントロール
    • Human liver tissue lysate and Rabex5 transfected 293T cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab69437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).

ターゲット情報

  • 機能
    Rab effector protein acting as linker between gamma-adaptin, RAB4A or RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates nucleotide exchange on RAB5A. Can act as a ubiquitin ligase.
  • 配列類似性
    Contains 1 A20-type zinc finger.
    Contains 1 VPS9 domain.
  • 翻訳後修飾
    Monoubiquitinated.
  • 細胞内局在
    Cytoplasm. Early endosome. Recycling endosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • RAB guanine nucleotide exchange factor (GEF) 1 antibody
    • Rab5 GDP/GTP exchange factor antibody
    • Rab5ef antibody
    • Rabaptin 5 associated exchange factor for Rab5 antibody
    • Rabaptin-5-associated exchange factor for Rab5 antibody
    • Rabex 5 antibody
    • Rabex-5 antibody
    • RABGEF 1 antibody
    • Rabgef1 antibody
    • RABX 5 antibody
    • RABX5 antibody
    • RABX5_HUMAN antibody
    • RAP1 antibody
    • Rin 2 antibody
    • Rin2 antibody
    see all

画像

  • Anti-Rabex5 antibody (ab69437) at 1/500 dilution + human liver tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size: 57 kDa
    Observed band size: 70 kDa (why is the actual band size different from the predicted?)

  • All lanes : Anti-Rabex5 antibody (ab69437) at 1/500 dilution

    Lane 1 : Rabex5 transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size: 57 kDa
    Observed band size: 57 kDa

プロトコール

参考文献

ab69437 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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