製品の概要

  • 製品名
    Rabbit specific HRP/AEC (ABC) Detection IHC Kit
    HRP/AEC Detection Kit キット 製品一覧
  • 製品の概要

    The reagents in this kit constitute a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.

  • 特記事項

    Contains hydrogen peroxide and Sodium azide.

  • アプリケーション
    適用あり: IHC-Pmore details

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 15 ml
    AEC Single Solution 1 x 15ml
    Biotinylated goat anti-rabbit IgG(H+L) 1 x 15ml
    Hydrogen Peroxide Block 1 x 15ml
    Protein block 1 x 15ml
    Streptavidin Peroxidase 1 x 15ml
  • 研究分野
  • 関連性
    An HRP/AEC Detection Kit is a labeled immunoenzymatic antigen detection system. Horseradish peroxidase (HRP) readily combines with hydrogen peroxide (H2O2) and the resultant complex can oxidize a wide variety of chromogenic substrates. AEC is 3-amino-9-ethyl-carbazole, a chromogenic substrate.
  • 別名
    • carbazole
    • Horseradish Peroxidase

アプリケーション

Our Abpromise guarantee covers the use of ab64260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent dilution.

画像

  • Human tonsil stained with anti-CD3 antibody using Anti Rabbit HRP/AEC Detection Kit (ab64260)

参考文献

This product has been referenced in:
  • Friedman O  et al. Immunological and inflammatory mapping of vascularized composite allograft rejection processes in a rat model. PLoS One 12:e0181507 (2017). IHC-P ; Rat . Read more (PubMed: 28746417) »

See 1 Publication for this product

レビューと Q&A

Thank you for replying with that information.

As I mentioned over the phone, I have spoken to the source of these kits and we do not have any experimental data using either the ACE or DABdetection kits (ab64260, ab64261, ab80437 and ab94361)f...

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Our ab93705 (Mouse and Rabbit Specific HRP/AEC (ABC) Detection IHC Kit) is not suitable for use on mouse samples. The secondary antibody is a cocktail of biotinylated anti-mouse and anti-rabbit antibodies and the anti-mouse antibodies will re...

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Thank you for contacting us. I can confirm the protein block has the following composition: PBS solution, pH 7.6 0.5% BSA 0.5% casein Less than 0.1% sodium azide I hope this information helps. Please do not hesitate to contact me shou...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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