50% glycerol, 0.1 M NaCl, 0.01 M sodium phosphate at pH 7.5.
Concentration information loading...
Immunogen affinity purified
Specific antibodies were absorpted by incubation with Sepharose-bound Sheep immunoglobulins.
Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis.
After repeated binding with immobilized Sheep immunoglobulins a minimum of 65% protein bound.
The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
F/P ratio is 3-5 mol Fluorescein per 1 mol of rabbit Ig.