Rabbit Anti-Sheep IgG H&L (Texas Red ®) (ab6745)

製品の概要

  • 製品名
    Rabbit Anti-Sheep IgG H&L (Texas Red ®)
    IgG 二次抗体 製品一覧
  • ターゲット生物種
    Sheep
  • アプリケーション
    適用あり: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISAmore details
  • 免疫原

    Sheep IgG whole molecule

  • 標識
    Texas Red ®. Ex: 596nm, Em: 620nm

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • 精製度
    IgG fraction
  • 特記事項(精製)
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 特記事項(標識)
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • ポリ/モノ
    ポリクローナル
  • 特記事項

    Texas RedTM Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 4.9 moles Texas Redä per mole of Rabbit IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/1000 - 1/5000. PubMed: 17895403
ELISA 1/10000 - 1/50000.

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  • Ab6745 was used at dilution 1/100 with the primary antibody ab30524 in ICC/IF. See the review on ab30524.

参考文献

This product has been referenced in:
  • Kirby BS  et al. Robust internal elastic lamina fenestration in skeletal muscle arteries. PLoS One 8:e54849 (2013). IHC . Read more (PubMed: 23359815) »
  • Pawlak E  et al. Distribution and processing of a disintegrin and metalloproteinase with thrombospondin motifs-4, aggrecan, versican, and hyaluronan in equine digital laminae. Am J Vet Res 73:1035-46 (2012). Read more (PubMed: 22738056) »

See all 4 Publications for this product

レビューと Q&A

This antibody has not been tested in sheep but is unlikely to cross-react as this polyclonal was raised in this species. The IgG concentration, however, should be in the region of 18.6mg/ml - as this is an IgG fraction, specific IgG will be approximate...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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