製品の概要

  • 製品名Rabbit Anti-Sheep IgG H&L (Biotin)
    IgG 二次抗体 製品一覧
  • ターゲット生物種Sheep
  • アプリケーション適用あり: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details
  • 免疫原

    Sheep IgG whole molecule

  • 標識Biotin

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 特記事項(標識)This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 特記事項

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000.
ELISA 1/140000.
Immunomicroscopy Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ICC/IF 1/500 - 1/3000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Rabbit Anti-Sheep IgG H&L (Biotin) 画像

  • Ab6746 was used at dilution 1/500 with the primary antibody ab9013 in IHC-P. See the review on ab9013.

Rabbit Anti-Sheep IgG H&L (Biotin) (ab6746) 使用論文

This product has been referenced in:
  • Thibault K  et al. BDNF-dependent plasticity induced by peripheral inflammation in the primary sensory and the cingulate cortex triggers cold allodynia and reveals a major role for endogenous BDNF as a tuner of the affective aspect of pain. J Neurosci 34:14739-51 (2014). IHC ; Rat . Read more (PubMed: 25355226) »
  • Periz J  et al. Rotavirus mRNAS are released by transcript-specific channels in the double-layered viral capsid. Proc Natl Acad Sci U S A 110:12042-7 (2013). Read more (PubMed: 23818620) »

See all 3 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab6746.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"