製品の概要

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー50% glycerol, 0.1 M NaCl, 0.01 M sodium phosphate at pH 7.5.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Specific antibodies were absorpted by incubation with Sepharose-bound rat immunoglobulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized rat immunoglobulins a minimum of 65% protein bound.
  • ポリ/モノポリクローナル
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab8519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
Dot blot
  • 追加情報Recommended dilution as secondary antibody in ELISA is 1:1000.
    Recommended dilution as secondary antibody in dot-blotting is 1:100.
  • Rabbit Anti-Rat IgG+IgM+IgA H&L (ab8519) 使用論文

    This product has been referenced in:
    • Lahoud MH  et al. Targeting antigen to mouse dendritic cells via Clec9A induces potent CD4 T cell responses biased toward a follicular helper phenotype. J Immunol 187:842-50 (2011). Read more (PubMed: 21677141) »

    See 1 Publication for this product

    Product Wall

    Thank you for contacting us.

    In general, the selection of the immunogen varies from antibody to antibody. Some antibodies are raised against synthetic peptides (based ona protein sequence which was derived originally from a DNA sequence), so...

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    I am sorry to hear that you have had problems with the 5 units of ab8519 that you purchased via VWR. the antibody has been tested in ELISA and Dot Blot only, not in an Ouchterlony gel. However I see no reason why the antibody should not work in this ap...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"