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Product name
Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP)
IgG 抗体 (1701件) 一覧
Target species
Goat
Tested applications
Dot Blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WBmore details
Immunogen
Goat IgG whole molecule
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Gentamicin Sulfate
Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, 0.01M Sodium phosphate, pH 7.2
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation notes
Horseradish Peroxidase (HRP)
Clonality
Polyclonal
Research areas
Secondary antibodies >> anti-Goat >> IgG >> Enzyme >> HRP
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab6741 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Dot: Use at an assay dependent dilution.
ELISA: 1/175000.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IM: Use at an assay dependent dilution.
ICC/IF: 1/1000 - 1/5000.
WB: 1/5000 - 1/20000. PubMed: 17369188
Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) images:
Western blot - Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab6741)
Ab6741 was used at dilution 1/5000 with the primary antibody ab23938 in WB. See the review on ab23938.
ELISA - Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab6741)
Ab6741 was used at dilution 1/1000 with the primary antibody ab2247 in ELISA. See the review on ab2247.
References for Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab6741)
This product has been referenced in:
- Pizzuto R et al. Pyruvate kinase in pig liver mitochondria. Arch Biochem Biophys 495:42-8 (2010). WB.Read more (PubMed: 20026031) »
- Ahn YH et al. Electrophilic tuning of the chemoprotective natural product sulforaphane. Proc Natl Acad Sci U S A 107:9590-5 (2010). WB.Read more (PubMed: 20439747) »
See all 12 publications for this product
Publishing research using ab6741? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"