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Products:Secondary antibodies >> anti-Chicken >> IgY >> Enzyme >> HRP
Overview
Product name
Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP)
IgY 抗体 (89件) 一覧
Target species
Chicken
Tested applications
ICC/IF, Dot Blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
Immunogen
chicken IgY whole molecule
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Gentamicin Sulfate (Do not add Sodium Azide!)
Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Horseradish Peroxidase (HRP).
Conjugation notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Clonality
Polyclonal
Isotype
IgG
Research areas
Secondary antibodies >> anti-Chicken >> IgY >> Enzyme >> HRP
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Immunohistochemistry (Frozen sections) - Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab6753)
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ELISA - Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab6753)
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Western blot - Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab6753)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab6753 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF
ICC/IF: 1/1000 - 1/5000.ICC/IF: 1/1000 - 1/5000.
Dot: Use at an assay dependent concentration.
ELISA: 1/145000
IHC-P: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration.
IM: Use at an assay dependent concentration.
WB: 1/2500 - 1/10000.
Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) images:
Immunohistochemistry (Frozen sections) - Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab6753)
Ab6753 was used at dilution 1/2000 with the primary antibody ab16293 in IHC-Fr. See the review on ab16293.
ELISA - Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab6753)
Ab6753 was used at dilution 1/10000 with the primary antibody ab15879 in ELISA. See the review on ab15879.
Western blot - Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab6753)
Ab6753 was used at dilution 1/5000 with the primary antibody ab19130 in WB. See the review on ab19130.
References for Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab6753)
This product has been referenced in:
- Vergnes Let al. Heart-type Fatty Acid-binding Protein Is Essential for Efficient Brown Adipose Tissue Fatty Acid Oxidation and Cold Tolerance. J Biol Chem 286:380-90 (2011). IHC-Fr.Read more (PubMed: 21044951) »
- Koshimizu Eet al. Embryonic senescence and laminopathies in a progeroid zebrafish model. PLoS One 6:e17688 (2011). WB.Read more (PubMed: 21479207) »
See all 12 publications for this product
Publishing research using ab6753? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"