The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/500. DyLight®550 replaces DyLight®549. The DyLight®550 excitation is 562nm and the emission is 576nm. DyLight®550 provides the same orange-to-red fluorescence and photostability as the DyLight®549 dye. Please refer to the vial in order to check whether the product you received is DyLight®549 or DyLight®550. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight550.
1/50 - 1/500.
1/50 - 1/200.
Use at an assay dependent concentration. See below Abreview
Western blot - Rabbit Anti-Mouse IgG H&L (DyLight® 550) preadsorbed (ab98786)This image is courtesy of an anonymous abreview.
All lanes : Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) at 1/1000 dilution
Lane 1 : Rat Hippocampus Lane 2 : Rat Hippocampus Lane 3 : Rat Hippocampus
Lysates/proteins at 25 µg per lane.
Secondary Lane 1 : Rabbit Anti-Mouse IgG H&L (DyLight® 550) preadsorbed (ab98786) at 1/5000 dilution Lane 2 : Rabbit Anti-Mouse IgG H&L (DyLight® 550) preadsorbed (ab98786) at 1/1000 dilution Lane 3 : Other Commercial Dye at 1/1000 dilution
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (DyLight® 550) preadsorbed (ab98786)This image is courtesy of an abreview submitted by Carl Hobbs, Kings's College London, United Kingdom.
Anti-HuC/D staining in mouse Formalin Fixed Paraffin Embedded sections. For antigen retrieval HIER in citrate buffer was performed. Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 550), pre-adsorbed (ab98786) was used as a secondary antibody at a concentration of 1/500.