Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97043)
Key features and details
- Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ICC, ELISA, IHC-P, WB
製品の概要
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製品名
Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase)
IgG 二次抗体 製品一覧 -
由来種
Rabbit -
ターゲット生物種
Mouse -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. -
アプリケーション
適用あり: ICC, ELISA, IHC-P, WBmore details -
標識
Alkaline Phosphatase
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, 0.58% Sodium chloride, 0.0095% Magnesium chloride, 0.00136% Zinc chloride, 1.19% HEPES -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP). -
特記事項(標識)
Molar enzyme: antibody protein ratio is 1:1 -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
Part of the AbExcel range. -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97043の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
Use at an assay dependent dilution.
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ELISA |
1/5000 - 1/50000. (Primary).
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|
IHC-P |
1/100 - 1/1000.
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|
WB |
1/5000 - 1/50000. (Chemiluminescent).
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特記事項 |
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ICC
Use at an assay dependent dilution. |
ELISA
1/5000 - 1/50000. (Primary). |
IHC-P
1/100 - 1/1000. |
WB
1/5000 - 1/50000. (Chemiluminescent). |
画像
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All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml
All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-2 : Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97043) at 1/5000 dilution
Lanes 3-4 : Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97043) at 1/20000 dilution
Lanes 5-6 : Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97043) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 20 minutes
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab97043 は 3 報の論文で使用されています。
- Gamon LF et al. Iodide modulates protein damage induced by the inflammation-associated heme enzyme myeloperoxidase. Redox Biol 28:101331 (2020). PubMed: 31568923
- Mao K et al. Potential regulation of glioma through the induction of apoptosis signaling via Egl-9 family hypoxia-inducible factor 3. Oncol Lett 13:893-897 (2017). PubMed: 28356975
- Bergeron KF et al. Male-biased aganglionic megacolon in the TashT mouse line due to perturbation of silencer elements in a large gene desert of chromosome 10. PLoS Genet 11:e1005093 (2015). IHC ; Mouse . PubMed: 25786024