Rabbit IgG, polyclonal - Isotype Control (ab27478)



  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: 1% BSA, 10 mM PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)ab27478 is a purified IgG. It was purified from serum collected from a rabbit prior to immunization.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野
  • 別名
    • rabbit isotype control


Our Abpromise guarantee covers the use of ab27478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration. PubMed: 20412779
IP Use at an assay dependent concentration. PubMed: 25078649
ICC/IF Use at an assay dependent concentration. PubMed: 17456778
IHC-P 1/200. PubMed: 23406280

Rabbit IgG, polyclonal - Isotype Control 画像

  • Positive immunostaining in brown color, counterstaining with haematoxylin (400× magnification). Only nuclear staining was considered positive. Hes1 is expressed in stage Sertoli and spermatogonia cells (B) using rabbit anti-Hes 1 (ab71559, diluted 1:100). Hes5 is expressed in Sertoli cells and elongated spermatids (D) using rabbit anti-Hes5 (ab25374, diluted 1:100). Control was done with rabbit IgG (A, C) (ab27478). Arrow heads point to spermatogonia cells. Arrows point to Sertoli cells. Tailed arrows point to elongated spermatids.

Rabbit IgG, polyclonal - Isotype Control (ab27478) 使用論文

This product has been referenced in:
  • Bae SY  et al. Immunohistochemical Localization of Translationally Controlled Tumor Protein in Axon Terminals of Mouse Hippocampal Neurons. Exp Neurobiol 26:82-89 (2017). IHC-P . Read more (PubMed: 28442944) »
  • Wang X  et al. Lyn kinase represses mucus hypersecretion by regulating IL-13-induced endoplasmic reticulum stress in asthma. EBioMedicine 15:137-149 (2017). ICC/IF . Read more (PubMed: 28024734) »

See all 39 Publications for this product

Product Wall

This Rabbit polyclonal is a purified antibody with a IgG concentration of 0.5MG/ML. Humans, mice, and rats have multiple IgG subclasses, whereas rabbits have only a single class of IgG. As this is a polyclonal the product is result of many differe...

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Thank you for your enquiry.

I can confirm that ab18414 can be stored at 4oC as described on the datasheet. This should not be aliquotted and frozen.

The others I would recommend to aliquot and freeze immediately. Keeping them at 4...

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I am very pleased to hear you would like to accept our offer and test ab54426 in IHC-P.


Expiration date: ######

This code will give you: 1 free primary antibody before the expiration date. To redee...

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Thank you for contacting us with your enquiry.

Ab27478 is a suitable isotype control to use with an unconjugated rabbit polyclonal primary antibody, so I would recommend using it as a negative controlfor ab100810 and ab7046 in IHC. As the IgG...

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Thank you for your reply.
Below are the 2 products that I would recommend for you:
Isotype control - http://www.abcam.com/Rabbit-IgG-ChIP-Grade-ab37415.html
Secondary - http://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H...

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Thank you for contacting Abcam.
I would be happy to help you select the best products for your experiments. To be able to assist you further, would you please let me know which applications you wish to use these products in. That way I can recommen...

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Thanks for your email.

You are welcome to use that credit code for a future purchase from Abcam. As you had requested a refund, please note that you can use the code to get a refund as well. Just forward this information on to your purchasing...

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Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to con...

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Thanks so much for the additional details. I apologize for the trouble with this antibody.

I believe you are using a reasonable protocol and that the vial of ab5506 you received is not working as expected. I can certainly issue a refund as yo...

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Thanks for your email and for providing the IHC images.

I'm sorry to hear this vial of ab5506 is giving you trouble. I'd just like to ask a couple additional questions:

1. What primary antibody dilutions have you tested?


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