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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FLISA plate was coated with Goat Anti-Rabbit IgG-UNLB and Goat Anti-Rabbit IgM-UNLB. Serially diluted Rabbit IgG-FITC ab37406 was captured and fluorescence intensity was quantified.
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Name: Rachel Dudek
Inquiry: Hello, I am wondering if you could provide me with the F/P ratio for antibody ab37406, a Rabbit IgG FITC - Isotype Control, so I can compare this to the F/P ratio of my specific antibody? The lot numb...
Asked on Nov 22 2013
Thank you very much for contacting us with your question, and for your patience.
I have contacted the lab scientists, and the F:P ratio is between 3 and 6 for this antibody.
I hope this information is useful, but please let me know if you h...
Abcam Scientific Support
Answered on Nov 22 2013
I have received today the product "Rabbit IgG FITC-Isotype Control (ab37406)" and I note that the storage instructions are changed.In fact, in the data sheet of the previous aliquots, the storage instructions reported: SHIPPED AT +4...
Asked on Jul 19 2013
We have done stability studies by storing conjugated antibodies at different temperature. The study shows FITC conjugated products are more stable at 4C rather than -20C so in response to this we changed the datasheets of all conjugated antibody we hav...
Abcam Scientific Support
Answered on Jul 19 2013
Inquiry: I have purchased the product with details below. Can you please provide me the concentration and the volume of the the product? What is your recommended concentration to use for flow cytometry using frozen peripheral blood mononuclear cell (PB...
Asked on Oct 04 2012
Thank you for contacting us and your interest in our products. The Rabbit IgG FITC - Isotype Control (ab37406) of the lot number GR59579-1 is provided at a concentration of 0.1mg/mL and is supplied as 1 mL. As the antibody is intend...
Abcam Scientific Support
Answered on Oct 04 2012
Hi, I have purchased ab37406 lot number GR77642. I need to use this alongside other antibodies at a known concentration. Please could you tell me the concentration of this antibody. Regards,
Asked on Sep 06 2012
Thank you for your inquiry. I am happy to confirm that ab37406 (lot GR77642) has a concentration of 0.1mg/ml. I hope this information is helpful and wish you good luck with your research.
Answered on Sep 06 2012
For the below antibodies I am looking for both isotype control and positive controls. It would be great if you could give the information for both in case you have them. ab36204 --> Rabbit IgG ab48614 --> Rabbit IgG ab66219 --> ...
Asked on Apr 03 2012
Thank you for your clarification. I can confirm the concentration of ab66219 (Batch: GR9812) of this ab is 1 mg/ml. The other antibody ab36204 (Lot: GR42382) is not affinity purified but whole antiserum so the IgG is not quantified. However, ...
Answered on Apr 03 2012
Thank you for the reply. In answer to your questions: 1. Unfortunately I have no data supporting the expression levels of the CDT1 in this particular cell line so it's definitely worthwhile to stain HeLa cells and see ...
Asked on Mar 29 2012
I think its good that you are checking the cell viability and using HeLa may provide more information as to if it is the cell strain or the antibodies that may be contributing to the problems. I agree that checking both the primary and secondary antibo...
Answered on Mar 29 2012
Hi xxxx, Firstly I'd like to thank you for all the suggestions you've made so far, it's been a very nice Abcam experience and the better technical support I've ever got. So, thank you again. You are right, now we now that there is...
Asked on Mar 14 2012
Thank you for providing such a full explanation of what you are trying to achieve. I can now understand exactly why you have chosen the technique that you have. I think the experiments you are planning to do next will be very useful in determ...
Answered on Mar 14 2012
Dear xxxx, I contacted you a few weeks ago as I'm having having some problems for stainng my samples with the following ICC system: Primary antibody: Anti-CDT1 antibody (ab83174) Secondary Antibody: Goat polyclonal Secondary Antibo...
Asked on Mar 13 2012
Thank you for getting back to me with your new results. It is good that we can now say conclusively that there is non-specific binding of both the secondary antibody and of the isotype control antibody. Following the protocol changes, I do no...
Answered on Mar 13 2012
Dear xxxx, The information provided is much appreciated, thank you very much for all the suggestions made. I think that definitely there are opportunities for improving my protocol. In answer to your question, the preparation of the dil...
Asked on Feb 16 2012
Thank you for that last bit of information. I just wanted to check that the isotype control wasn't being used in a higher concentration than the primary antibody, but everything is fine. I look forward to hearing how you...
Answered on Feb 16 2012
Dear xxxx, Please find attached to this email the images corresponding to both, the positve staining controls and the actual controls for discarding misbinding of the antibodies. Even thouh I'm showing just one set of images the general...
Sorry for the delay in getting back to you and thank you for all that information. It makes it much easier to understand what is going on and what has been tried previously. I think the next step you are suggestingare good. The blocking buffe...
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