Rabbit Anti-Human IgA H&L (HRP) (ab8510)
Key features and details
- Rabbit Anti-Human IgA H&L (HRP)
- Conjugation: HRP
- Host species: Rabbit
- Suitable for: Dot blot, ELISA
製品の概要
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製品名
Rabbit Anti-Human IgA H&L (HRP)
IgA 二次抗体 製品一覧 -
由来種
Rabbit -
ターゲット生物種
Human -
特異性
This product does have a minor cross-reactivity to mouse samples.
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アプリケーション
適用あり: Dot blot, ELISAmore details -
標識
HRP
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. -
バッファー
pH: 7.50
Constituents: 0.164% Sodium phosphate, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose-bound human IgM and IgG. Specific antibodies were absorpted by incubation with Sepharose-bound human IgA. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgA a minimum of 65% protein bound. -
特記事項(標識)
The purified polyclonal was conjugated to horseradish peroxidase according to the periodate method followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate. -
ポリ/モノ
ポリクローナル -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8510の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Dot blot |
1/100.
|
|
ELISA |
1/1000.
|
特記事項 |
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Dot blot
1/100. |
ELISA
1/1000. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab8510 は 3 報の論文で使用されています。
- Lutomski CA et al. Multiple Roles of SARS-CoV-2 N Protein Facilitated by Proteoform-Specific Interactions with RNA, Host Proteins, and Convalescent Antibodies. JACS Au 1:1147-1157 (2021). PubMed: 34462738
- Kempsell KE et al. Whole genome protein microarrays for serum profiling of immunodominant antigens of Bacillus anthracis. Front Microbiol 6:747 (2015). ELISA . PubMed: 26322022
- Csorba K et al. Development of an ELISA for sensitive and specific detection of IgA autoantibodies against BP180 in pemphigoid diseases. Orphanet J Rare Dis 6:31 (2011). ELISA . PubMed: 21619684