製品の概要

  • 製品名
    Rabbit Anti-Human IgA H&L (FITC)
    IgA 二次抗体 製品一覧
  • ターゲット生物種
    Human
  • 特異性
    Antibodies were raised in rabbit to human IgA
  • アプリケーション
    適用あり: ELISA, Dot blotmore details
  • 標識
    FITC. Ex: 493nm, Em: 528nm

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    50% glycerol, 0.1 M NaCl, 0.01 M sodium phosphate at pH 7.5.
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose-bound human IgG and IgM. Specific antibodies were absorpted by incubation with Sepharose-bound human IgA. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgA a minimum of 65% protein bound.
  • 特記事項(標識)
    The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
  • ポリ/モノ
    ポリクローナル
  • 特記事項
    F/P ratio is 4-6 mol Fluorescein per 1 mol of rabbit IgA. There is low background fluorescence in normal tissue in contrast to cases of autoimmune desease where IgA is depostited.
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab8509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
Dot blot
  • 追加情報
    Recommended dilution as secondary antibody in ELISA is 1:1000.
    Recommended dilution as secondary antibody in dot-blotting is 1:100.
  • 参考文献

    ab8509 has not yet been referenced specifically in any publications.

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