Rabbit Anti-Human IgA alpha chain (FITC) (ab97219)

製品の概要

  • 製品名
    Rabbit Anti-Human IgA alpha chain (FITC)
    IgA 二次抗体 製品一覧
  • ターゲット生物種
    Human
  • 特異性
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgA. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
  • アプリケーション
    適用あり: ICC/IF, IHC-P, Flow Cytmore details
  • 標識
    FITC. Ex: 493nm, Em: 528nm

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to FITC.
  • 特記事項(標識)
    F/P ratio is 3-7
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Rabbit Anti-Human IgA alpha chain (FITC) (ab97219) 使用論文

ab97219 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application
Flow Cytometry
Background: We wanted to distinguish human B cells into IgM+, IgG+ and IgA+ B cells by staining surface immunoglobulin. Since ab97219 is polyclonal, presence of an antibody targeting the extracellular part of the IgA heavy chain could be suspected but needed to be tested.
Staining: Human peripheral blood-derived PBMC stained with antibodies against CD3, CD19, surface IgG and surface IgA. In the image, lymphocytes are gated on CD3- CD19+
Results: There is a clear population of IgA+ B cells in the expected range. A few possibly IgA+/IgG+ B cells are present too.
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Mr. Marc Bigler

Verified customer

投稿 Aug 18 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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