Rabbit Anti-Human IgA alpha chain (ab97216)

製品の概要

  • 製品名
    Rabbit Anti-Human IgA alpha chain
    IgA 二次抗体 製品一覧
  • ターゲット生物種
    Human
  • 特異性
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgA. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
  • アプリケーション
    適用あり: IHC-P, ELISA, WB, ICCmore details

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/200 - 1/2000.
ELISA 1/100 - 1/30000. Coating: 1/100 - 1/500; Primary: 1/1000 - 1/30000
WB 1/1000 - 1/30000. Colorimetric: 1/1000 - 1/10000; Chemiluminescent: 1/1000 - 1/30000
ICC Use at an assay dependent dilution.

参考文献

ab97216 has not yet been referenced specifically in any publications.

レビューと Q&A

Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Positive control MY5 and negative control LP-1 (multiple myeloma) cell lines immersion-fixed in 4% PFA, permeablized, blocked in 10% donkey serum/PBS 1 hour and incubated with 1/2000 ab97216 in 10% donkey serum/PBS overnight at 4oC. Washed 3X 5 minutes PBS and incubated with 1/2000 Alexa 488 donkey anti-rabbit F(ab2)" secondary antibody (Jackson). Washed 5X 5 minutes PBS and mounted in DAPI/Vectashield. Images acquired on Zeiss AxioImager FITC channel at 50ms exposure.
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Ms. Natalie Erdmann

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投稿 Sep 21 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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