Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) preadsorbed (ab7133)

製品の概要

  • 製品名Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) preadsorbed
    IgG 二次抗体 製品一覧
  • ターゲット生物種Goat
  • アプリケーション適用あり: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IFmore details
  • 吸着処理血清
    Human more details
  • 免疫原

    Goat IgG whole molecule

  • 標識Alkaline Phosphatase

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.60
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.79% Tris HCl, 0.0095% Magnesium chloride, 0.0014% Zinc chloride, 50% Glycerol
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads.
  • 特記事項(標識)Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • ポリ/モノポリクローナル
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Dot blot Use at an assay dependent concentration.
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
WB Use at an assay dependent dilution.
ICC/IF 1/200 - 1/1000.

Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) preadsorbed (ab7133) 使用論文

ab7133 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. 1. How much lysate was loaded? We would suggest 5uL for 10x8cm gel. 2. Try to titer up the secondary antibody. The problem seems like a non-specific binding caused by too much antigen and/or antibody.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"