Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) (ab97097)
Key features and details
- Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
製品の概要
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製品名
Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase)
IgG 二次抗体 製品一覧 -
由来種
Rabbit -
ターゲット生物種
Goat -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. -
アプリケーション
適用あり: ICC, IHC-P, ELISA, WBmore details -
標識
Alkaline Phosphatase
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, 0.58% Sodium chloride, 0.0095% Magnesium chloride, 0.00136% Zinc chloride, 1.19% HEPES -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP). -
特記事項(標識)
Molar enzyme: antibody protein ratio is 1:1 -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
Part of the AbExcel range. -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97097の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC |
Use at an assay dependent dilution.
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IHC-P |
1/100 - 1/1000.
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|
ELISA |
1/5000 - 1/50000. (Primary).
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|
WB |
1/5000 - 1/50000. (Chemiluminescent).
|
特記事項 |
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ICC
Use at an assay dependent dilution. |
IHC-P
1/100 - 1/1000. |
ELISA
1/5000 - 1/50000. (Primary). |
WB
1/5000 - 1/50000. (Chemiluminescent). |
画像
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All lanes : Anti-beta Tubulin antibody (ab21057) at 1 µg/ml
All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-2 : Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) (ab97097) at 1/5000 dilution
Lanes 3-4 : Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) (ab97097) at 1/20000 dilution
Lanes 5-6 : Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) (ab97097) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 20 minutes
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab97097 は 2 報の論文で使用されています。
- Trainor BM et al. Distinct requirements for the COMPASS core subunits Set1, Swd1, and Swd3 during meiosis in the budding yeast Saccharomyces cerevisiae. G3 (Bethesda) 11:N/A (2021). PubMed: 34849786
- Millar SA et al. An Investigation Into the Role of Osteocalcin in Human Arterial Smooth Muscle Cell Calcification. Front Endocrinol (Lausanne) 11:369 (2020). PubMed: 32587575