Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) (ab97097)

製品の概要

  • 製品名Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase)
    IgG 二次抗体 製品一覧
  • ターゲット生物種Goat
  • 特異性By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • アプリケーション適用あり: ICC, IHC-P, ELISA, WBmore details
  • 標識Alkaline Phosphatase

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
  • 特記事項(標識)Molar enzyme: antibody protein ratio is 1:1
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 特記事項Part of the AbExcel range.
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent dilution.
IHC-P 1/100 - 1/1000.
ELISA 1/5000 - 1/50000. (Primary).
WB 1/5000 - 1/50000. (Chemiluminescent).

Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) 画像

  • All lanes : Anti-beta Tubulin antibody - Loading Control (ab21057) at 1 µg/ml and HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg per lane. 

    Secondary
    Lanes 1 - 2 : Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97097) at 1/5000 dilution
    Lanes 3 - 4 : Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97097) at 1/20000 dilution
    Lanes 5 - 6 : Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97097) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time : 20 minutes

Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) (ab97097) 使用論文

ab97097 has not yet been referenced specifically in any publications.

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Thank you for your inquiry. I am happy to confirm that all these products are suitable for WB and tested and guaranteed.
I suggest ab29889 (Liver (Human) Tissue Lysate - adult normal tissue) as positive control for ab3366, ab3373, ab3375 and ab241...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"