Rabbit F(ab')2 Anti-Rat IgG - H&L (HRP), pre-adsorbed (ab6250)

製品の概要

  • 製品名
    Rabbit F(ab')2 Anti-Rat IgG - H&L (HRP), pre-adsorbed
    IgG 二次抗体 製品一覧
  • ターゲット生物種
    Rat
  • 特異性
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
  • アプリケーション
    適用あり: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details
  • 吸着処理血清

    Cow, Horse, Human, Mouse, Rabbit, Sheep more details
  • 免疫原

    Rat IgG, whole molecule

  • 標識
    HRP

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 特記事項(精製)
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 特記事項(標識)
    Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
Dot blot
WB
ELISA
Electron Microscopy
IHC
  • 追加情報
    Dot: Use at an assay dependent dilution.
    Electron Microscopy: Use at an assay dependent dilution.
    ELISA: 1/3000 - 1/12000.
    IHC: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.

    This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/3000 to 1/12000 of the reconstitution concentration is suggested for this product.

    Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 参考文献

    ab6250 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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