By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Biotin.