This antibody reacts with Goat heavy and light chains. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against anti-pepsin.
Full length (Goat) IgG whole molecules.
FITC. Ex: 493nm, Em: 528nm
Shipped at 4°C. Store at +4°C.
Preservative: 0.01% Sodium Azide
Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
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Immunogen affinity purified
Prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
4.1 moles FITC per mole of Rabbit IgG F(ab')
Label: Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons)
Absorption Wavelength: 495 nm; Emission Wavelength: 528 nm.
This antibody is suitable for antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000.
1/500 - 1/2500.
Rabbit F(ab')2 Anti-Goat IgG H&L (FITC) (ab47846) 使用論文
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"