Rabbit F(ab')2 Anti-Goat IgG - H&L (Biotin), pre-adsorbed (ab102348)

製品の概要

  • 製品名
    Rabbit F(ab')2 Anti-Goat IgG - H&L (Biotin), pre-adsorbed
    IgG 二次抗体 製品一覧
  • ターゲット生物種
    Goat
  • 特異性
    By immunoelectrophoresis and ELISA this antibody reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human, mouse and rat IgG was detected. This antibody may cross react with IgG from other species.
  • アプリケーション
    適用あり: IHC-P, ICC, ELISA, WBmore details
  • 吸着処理血清

    Human, Mouse, Rat more details
  • 標識
    Biotin

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Antiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Biotin.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab102348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/500 - 1/5000.
ICC 1/200 - 1/1000.
ELISA 1/10000 - 1/200000. (Primary).
WB 1/10000 - 1/200000. (Chemiluminescent).

参考文献

ab102348 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Secondary Ab: Biotinylated rabbit anti-Goat F(ab’)2 (Abcam ab102348) Concentration 1:500 30’ RT
Labeling system : Avidin-biotin complex (Vector Lab, Vectastain Elite ABC kit PK-6100) 30’ RT
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投稿 Jul 24 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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