Specific antibodies were absorpted by incubation with Sepharose-bound chicken. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized chicken immunoglobulins a minimum of 65% protein bound.
The purified polyclonal was labeled with fluorescein isothiocyanate isomer I followed by gel-filtation and ion-exchange chromatography to clear unbound conjugate.
F/P ratio: 3-5 mol Fluorescein per 1 mol rabbit IgG.