製品の概要

  • 製品名Rabbit Anti-Chicken IgY H&L (Biotin)
    IgY 二次抗体 製品一覧
  • ターゲット生物種Chicken
  • アプリケーション適用あり: IHC-Fr, Immunomicroscopy, WB, Dot blot, ELISA, IHC-P, ICC/IFmore details
  • 免疫原

    chicken IgY whole molecule

  • 標識Biotin

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule.
  • 特記事項(標識)This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • ポリ/モノポリクローナル
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
WB 1/2000 - 1/20000.
Dot blot Use at an assay dependent concentration.
ELISA 1/500000.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/1000 - 1/5000.

Rabbit Anti-Chicken IgY H&L (Biotin) 画像

  • Ab6752 was used with the primary antibody ab13989 in ICC/IF. See Abreview on ab13989.
  • Ab6752 was used at dilution 1/1000 with the primary antibody ab13970 in IHC-Fr. See the review on ab13970.

Rabbit Anti-Chicken IgY H&L (Biotin) (ab6752) 使用論文

This product has been referenced in:
  • Weis B  et al. Inhibition of intestinal tumor formation by deletion of the DNA methyltransferase 3a. Oncogene 0:N/A (2014). Read more (PubMed: 24837369) »
  • Jiao TT  et al. Importance of spondin 1 and cellular retinoic acid binding protein 1 in the clinical diagnosis of ovarian cancer. Int J Clin Exp Pathol 6:3036-41 (2013). IHC-P . Read more (PubMed: 24294399) »

See all 6 Publications for this product

Product Wall

Thank you for your enquiry. Antibodies can be stored with glyserol at -20oC. However, there is some evidence that this can sometimes decrease the activity of the antibody which means you may have to use the antibody at a higher concentration in your ex...

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We recommend the following dilutions: ELISA 1:5,000 - 1:20,000 Immunoblotting 1:3,000 - 1:10,000 Immunohistochemistry 1:100 - 1:1,000

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"