製品の概要

  • 製品名Anti-Rab9 antibody
    Rab9 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Rab9
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Rab9.

    (Peptide available as ab45894.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates: Spleen (Mouse) and Spleen (Rat).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab45864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).

ターゲット情報

Anti-Rab9 antibody 画像

  • All lanes : Anti-Rab9 antibody (ab45864) at 1 µg/ml

    Lane 1 : Mouse spleen tissue lysate - total protein (ab29293)
    Lane 2 : Spleen (Rat) Tissue Lysate - normal tissue (ab29393)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 23 kDa
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
  • IHC image of ab45864 staining in mouse spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45864, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Rab9 antibody (ab45864) 使用論文

ab45864 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab45864.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"