製品の概要

  • 製品名
    Anti-RAB6A antibody
  • 製品の詳細
    Rabbit polyclonal to RAB6A
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Zebrafish
  • 免疫原

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 178 of RAB6A

  • ポジティブ・コントロール
    • H1299 whole cell lysate HeLa cells MDA-MB-468 xenograft tissue

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab95954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 24 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

ターゲット情報

Anti-RAB6A antibody 画像

  • Anti-RAB6A antibody (ab95954) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size : 24 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab95954 at 1:200 dilution (1) and merged with DNA probe (2).
  • ab95954, at a 1:500 dilution, staining human RAB6A in MDA-MB 468 xenograft, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.

Anti-RAB6A antibody (ab95954) 使用論文

This product has been referenced in:
  • Petrosyan A  et al. Restoration of compact Golgi morphology in advanced prostate cancer enhances susceptibility to galectin-1-induced apoptosis by modifying mucin O-glycan synthesis. Mol Cancer Res 12:1704-16 (2014). Read more (PubMed: 25086069) »
  • Petrosyan A & Cheng PW A non-enzymatic function of Golgi glycosyltransferases: mediation of Golgi fragmentation by interaction with non-muscle myosin IIA. Glycobiology 23:690-708 (2013). Read more (PubMed: 23396488) »

See all 2 Publications for this product

Product Wall

Application
Western blot
Loading amount
35 µg
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Sample
Rat Cell lysate - other (Rat hepatocytes plasma membrane fraction)
Specification
Rat hepatocytes plasma membrane fraction
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

投稿 Oct 29 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample
Rat Cell (Rat hepatocytes)
Specification
Rat hepatocytes
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

投稿 Oct 28 2014

Application
Western blot
Loading amount
35 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (LNCaP prostate cancer cells)
Specification
LNCaP prostate cancer cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

投稿 Nov 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Panc-1)
Specification
Panc-1
Fixative
Formaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

投稿 Dec 03 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録