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Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Fruit fly (Drosophila melanogaster) Rab5.
(Peptide available as ab32489.)
Our Abpromise guarantee covers the use of ab31261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 20404143|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).|
ab31261 staining 3rd instar wing imaginal disc cells of fruit fly (Drosophila melanogaster). Cells were formaldehyde fixed and blocked with 5 % serum for 2 hours at 22°C prior to incubating with the primary antibody, diluted 1/500, for 16 hours at 4°C. A Cy3® conjugated donkey anti-rabbit IgG (H+L) antibody, diluted 1/500, was used as the secondary.
In the image, the nuclei of some cells are marked by GFP (green). The punctate, non-nuclear staining pattern is indicative of endosomes (similar to other endosomal markers such as Hrs).