製品の概要

  • 製品名
    Anti-Rab5 antibody [3A4]
    Rab5 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3A4] to Rab5
  • アプリケーション
    適用あり: WB, ELISA, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant fragment corresponding to amino acids 1-215 of Human Rab5 purified from E. coli.

  • ポジティブ・コントロール
    • Extracts of mouse brain.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab66746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 2-5µg for 106 cells.

ターゲット情報

画像



  • Predicted band size : 24 kDa

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: Rab5 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: Hu brain whole cell lysate (20 µg)
    Lane 4: Ms brain whole cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab66746 observed at 25 kDa. Red - loading control, ab181602, observed at 37 kDa.

  • ab66746 staining Rab5 in the U87MG human cell line at a dilution of 1/100. 

    DAPI was used for nuclear staining.

  • Anti-Rab5 antibody [3A4] (ab66746) at 1/500 dilution + extracts of mouse brain at 45 µg

    Secondary
    HRP-conjugated goat anti-mouse
    Developed using the ECL technique

    Predicted band size : 24 kDa
    Observed band size : 24 kDa
  • Flow cytometry analysis of ab66746 staining Rab5 in HeLa cells. Primary antibody was used at a concentration of 2-5ug per 1 X 106 cells. The secondary antibody used was an Alexa fluor 488® conjugated goat anti-mouse IgG.

参考文献

This product has been referenced in:
  • Del Cid-Pellitero E  et al. Homeostatic Changes in GABA and Glutamate Receptors on Excitatory Cortical Neurons during Sleep Deprivation and Recovery. Front Syst Neurosci 11:17 (2017). Read more (PubMed: 28408870) »

See 1 Publication for this product

レビューと Q&A

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 6% · Temperature: 20°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 09 2016

Thank you for your email.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab66746.
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Thank you for contacting us.

Yes, I'd be happy to send you one of the mouse antibodies, but am afraid I cannot make the final decision for you. I'd be able to make a recommendation, though.
Both antibodies (ab66746,ab55657) have not bee...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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