製品の概要

  • 製品名
    Anti-RAB40B antibody
  • 製品の詳細
    Rabbit polyclonal to RAB40B
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 144-193 (YAERLGVTFF EVSPLCNFNI TESFTELARI VLLRHGMDRL WRPSKVLSLQ) of Human RAB40B (NP_006813).

  • ポジティブ・コントロール
    • 293T cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab82896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/12500.

ターゲット情報

  • 関連性
    RAB40B is similar to a yeast protein which suggests a role of the gene product in regulating secretory vesicles. It may be a substrate recognition component of a SCF like ECS (Elongin Cullin SOCS box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
  • 細胞内局在
    Cell membrane; Lipid anchor; Cytoplasmic side
  • 参照データベース
  • 別名
    • FLJ42385 antibody
    • GTP binding protein homologous to Saccharomyces cerevisiae SEC4 antibody
    • RAB40B member RAS oncogene family antibody
    • RAR antibody
    • Rar protein antibody
    • Ras related protein Rab 40B antibody
    • RP23 293H17.2 antibody
    • SEC4L antibody
    • SOCS box containing protein RAR antibody
    see all

画像

  • Anti-RAB40B antibody (ab82896) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 31 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

ab82896 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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