Anti-Rab4 抗体 [EPR3043] - Early Endosome Marker (ab109009)


  • 製品名Anti-Rab4 antibody [EPR3043] - Early Endosome Marker
    Rab4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3043] to Rab4 - Early Endosome Marker
  • アプリケーション適用あり: WB, IP, ICC/IFmore details
    適用なし: Flow Cyt or IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Rab4 aa 150-250 (C terminal).

  • ポジティブ・コントロール
    • MCF7, PC12, Neuro 2a, 293T, SH SY5Y and Human fetal brain lysates; HeLa cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.



Our Abpromise guarantee covers the use of ab109009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
IP 1/10 - 1/100.
ICC/IF 1/250 - 1/1000.
  • 追加情報Is unsuitable for Flow Cyt or IHC-P.
  • ターゲット情報

    Anti-Rab4 antibody [EPR3043] - Early Endosome Marker 画像

    • Predicted band size : 24 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Rab4 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Human fetal brain lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab109009 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab109009 was shown to specifically react with Rab4 when Rab4 knockout samples were used. Wild-type and Rab4 knockout samples were subjected to SDS-PAGE. ab109009 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW)  preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD)  preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labelling Rab4 with purified ab109009 at 1/250. Cells were fixed with 100% methanol and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

    • All lanes : Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : PC12 cell lysate
      Lane 3 : Neuro 2a cell lysate
      Lane 4 : 293T cell lysate
      Lane 5 : SH SY5Y cell lysate
      Lane 6 : Human fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 24 kDa
    • ab109009 at 1/500 dilution staining Rab4 in HeLa by Immunofluorescence.

    Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009) 使用論文

    ab109009 has not yet been referenced specifically in any publications.

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