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Synthetic peptide corresponding to residues in Human Rab4.
Our Abpromise guarantee covers the use of ab108974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa).|
|Flow Cyt||1/150. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.|
|ICC/IF||1/100 - 1/250.|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Rab4 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Human fetal brain cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab108974 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab108974 was shown to recognize Rab4 when Rab4 knockout samples were used, along with additional cross-reactive bands. Wild-type and Rab4 knockout samples were subjected to SDS-PAGE. ab108974 and ab8245 (loading control to GAPDH) were both diluted 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
ab108974 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"