Anti-Rab4 抗体 - Early Endosome Marker (ab13252)
Key features and details
- Rabbit polyclonal to Rab4 - Early Endosome Marker
- Suitable for: WB, ICC
- Reacts with: Rat
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Rab4 antibody - Early Endosome Marker
Rab4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Rab4 - Early Endosome Marker -
由来種
Rabbit -
特異性
This antibody is very specific for Rab4. To our knowledge this antibody does not cross-react with other Rab proteins. However, as there are dozens of different proteins in the Rab family, not every single one has been tested for cross-reactivity with this antibody. -
アプリケーション
適用あり: WB, ICCmore details -
種交差性
交差種: Rat -
免疫原
Fusion protein corresponding to Human Rab4.
Database link: P20338 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13252の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 25.6 kDa).
|
ICC | (1) |
1/200 - 1/500.
|
特記事項 |
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WB
1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 25.6 kDa). |
ICC
1/200 - 1/500. |
ターゲット情報
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機能
Protein transport. Probably involved in vesicular traffic. -
配列類似性
Belongs to the small GTPase superfamily. Rab family. -
翻訳後修飾
Phosphorylated by CDK1 kinase during mitosis. -
細胞内局在
Membrane. Cytoplasm. Generally associated with membranes. Cytoplasmic when phosphorylated by CDK1. - Information by UniProt
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参照データベース
- Entrez Gene: 25532 Rat
- SwissProt: P05714 Rat
- Unigene: 3016 Rat
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別名
- HRES 1 / RAB4 antibody
- Oncogene RAB4 antibody
- Rab 4 antibody
see all
画像
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ab13252, rabbit polyclonal to Rab4 was used in fixed primary cultures of rat hippocampal neurons (embryonic day 18) at a dilution of 1/200. A secondary goat anti-rabbit antibody was used for detection (Alexa Fluor 488; 1/200; green). Confocal microscopy revealed typically punctuate immunofluorescence expected of Rab4 in these neurons. The red colour labelling is that of monoclonal mouse anti-beta-tubulin II at a 1/400 dilution, followed by secondary goat anti-mouse Alexa Fluor 568.
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Anti-Rab4 antibody - Early Endosome Marker (ab13252) at 1/1000 dilution + Lysates prepared from rat brain tissue
Predicted band size: 25.6 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (36)
ab13252 は 36 報の論文で使用されています。
- Giridharan SSP et al. Lipid kinases VPS34 and PIKfyve coordinate a phosphoinositide cascade to regulate retriever-mediated recycling on endosomes. Elife 11:N/A (2022). PubMed: 35040777
- Pazzaglia S et al. Out-of-Field Hippocampus from Partial-Body Irradiated Mice Displays Changes in Multi-Omics Profile and Defects in Neurogenesis. Int J Mol Sci 22:N/A (2021). PubMed: 33924260
- Actis Dato V & Chiabrando GA Activated Alpha-2 Macroglobulin Improves Insulin Response via LRP1 in Lipid-Loaded HL-1 Cardiomyocytes. Int J Mol Sci 22:N/A (2021). PubMed: 34203120
- Souza ILM et al. Endocytosis of the non-catalytic ADAM23: Recycling and long half-life properties. Exp Cell Res 398:112415 (2021). PubMed: 33296662
- Khera L et al. The AXL-PYK2-PKCa axis as a nexus of stemness circuits in TNBC. Life Sci Alliance 4:N/A (2021). PubMed: 33785524