製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RAB3IP
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 36-85 (PDLLGVYESG TQEQTTSPSV IYRPHPSALS SVPIQANALD VSELPTQPVY) of Human RAB3IP (NP_783322).

  • ポジティブ・コントロール
    • 293T cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab90732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/200.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Guanine nucleotide exchange factor (GEF) which may activate RAB8A and RAB8B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. Mediates the release of GDP from RAB8A and RAB8B but not from RAB3A or RAB5. Modulates actin organization and promotes polarized transport of RAB8A-specific vesicles to the cell surface. Together with RAB11A, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis.
  • 組織特異性
    Expressed in brain, kidney, heart, pancreas and placenta. Not detected in skeletal muscle or liver.
  • 配列類似性
    Belongs to the SEC2 family.
  • 細胞内局在
    Cytoplasm. Nucleus. Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Predominantly cytoplasmic but a small proportion colocalizes with SSX2 in the nucleus. Activation of protein kinase C results in redistribution to the periphery of lamellipodia. In the cytoskeleton, localizes to cortical actin.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Gtpat12 antibody
    • Rab-3A-interacting protein antibody
    • RAB3A interacting protein antibody
    • RAB3A interacting protein (rabin3) antibody
    • RAB3A interacting protein antibody
    • RAB3A-interacting protein antibody
    • RAB3I_HUMAN antibody
    • Rab3ip antibody
    • Rabin 3 antibody
    • Rabin-3 antibody
    • RABIN3 antibody
    • RABIN3, rat, homolog of antibody
    • Rabin8 antibody
    • SSX2 interacting protein antibody
    • SSX2 interacting protein antibody
    • SSX2-interacting protein antibody
    see all

画像

  • Primary Antibody Dilution : 1:200. Secondary Antibody : Goat anti-rabbit Alexafluor 568. Secondary Antibody Dilution : 1:200. Color/Signal Descriptions : RAB3IP: Red CtBp: Green DAPI: Blue. Gene Name : RAB3IP.
  • Primary Antibody Dilution : 1:200. Secondary Antibody : Goat anti-rabbit Alexafluor 568. Secondary Antibody Dilution : 1:200. Color/Signal Descriptions : RAB3IP: Red CtBp: Green DAPI: Blue. Gene Name : RAB3IP.
  • Primary Antibody Dilution : 1:200. Secondary Antibody : Goat anti-rabbit Alexafluor 568. Secondary Antibody Dilution : 1:200. Color/Signal Descriptions : RAB3IP: Red CtBp: Green DAPI: Blue. Gene Name : RAB3IP.
  • Anti-RAB3IP antibody (ab90732) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 53 kDa
    Gel concentration: 12%

参考文献

This product has been referenced in:
  • Liu Y  et al. KRASG12 mutant induces the release of the WSTF/NRG3 complex, and contributes to an oncogenic paracrine signaling pathway. Oncotarget 7:53153-53164 (2016). Read more (PubMed: 27449290) »

See 1 Publication for this product

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