製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Rab3C
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Cow
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 1-197 of Human Rab3C (NP_612462).

  • ポジティブ・コントロール
    • H1299 whole cell lysate; DLD1 xenograft; 293T, A431, HeLa and HepG2 cell lysates.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab96360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 26 kDa.
IHC-P 1/100 - 1/500.

ターゲット情報

画像

  • Anti-Rab3C antibody (ab96360) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size: 26 kDa



    12% SDS PAGE
  • ab96360, at 1/500 dilution, staining Rab3C in paraffin-embedded DLD1 xenograft by Immunohistochemistry.

参考文献

ab96360 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat hepatocytes)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
30 µg
Specification
Rat hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

投稿 Mar 22 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HepG2 cells)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
HepG2 cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

投稿 Mar 22 2018

Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2 cells)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
20 µg
Specification
HepG2 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

投稿 Mar 22 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

投稿 Mar 20 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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