Rabbit polyclonal to RAB39
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse RAB39.
This antibody gave a positive signal in the following lysates:
Spleen (Mouse) Tissue Lysate; WI38 Whole Cell Lysate; A549 Whole Cell Lysate; HeLa Whole Cell Lysate
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
May be involved in vesicular trafficking.
Belongs to the small GTPase superfamily. Rab family.
Information by UniProt
rab related GTP binding protein antibody
Western blot - RAB39 antibody (ab99445)
All lanes : Anti-RAB39 antibody (ab99445) at 1 µg/ml Lane 1 : Spleen (Mouse) Tissue Lysate Lane 2 : WI38 (Human lung fibroblast cell line) Whole Cell Lysate Lane 3 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane. Secondary Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution Developed using the ECL technique Performed under reducing conditions. Predicted band size : 25 kDa Observed band size : 25 kDa Additional bands at : 20 kDa,250 kDa. We are unsure as to the identity of these extra bands. Exposure time : 20 minutes
has not yet been referenced specifically in any publications.
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