製品の概要

  • 製品名Anti-RAB30 antibody
    RAB30 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to RAB30
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Recombinant Fragment
    交差が予測される動物種: Human
  • 免疫原

    Recombinant full length human RAB30 protein (AAH14213) with tag.

  • ポジティブ・コントロール
    • Recombinant tagged full length human RAB30.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab76622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 23 kDa.

    This antibody has only been tested in WB against the recombinant protein used as immunogen. We have no data on the detection of endogenous protein.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 配列類似性Belongs to the small GTPase superfamily. Rab family.
    • 細胞内局在Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 5033421K01Rik antibody
      • AI323892 antibody
      • OTTMUSP00000025677 antibody
      • OTTMUSP00000025678 antibody
      • RAB 30 antibody
      • Rab30 antibody
      • RAB30 member RAS oncogene family antibody
      • RAB30_HUMAN antibody
      • Ras related protein Rab 30 antibody
      • Ras-associated protein Rab30 antibody
      • Ras-related protein Rab-30 antibody
      • Rsb30 antibody
      see all

    Anti-RAB30 antibody 画像

    • Anti-RAB30 antibody (ab76622) at 1 µg/ml + Recombinant tagged full length human RAB30 at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

      Predicted band size : 23 kDa
      Observed band size : 48 kDa (why is the actual band size different from the predicted?)

    Anti-RAB30 antibody (ab76622) 使用論文

    ab76622 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (A549 lung epithelial)
    Specification A549 lung epithelial
    Fixative 4% formaldehyde
    Permeabilization Yes - 0.2% triton x100 in PBS
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 18°C
    Username

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    投稿 Nov 16 2011

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (A549 lung epithelial)
    Loading amount 20000 cells
    Specification A549 lung epithelial
    Treatment siRNA treated
    Gel Running Conditions Reduced Denaturing (15%)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 18°C
    Username

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    Verified customer

    投稿 Nov 16 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"