製品の概要

  • 製品名Anti-Rab25 antibody [3F12]
    Rab25 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3F12] to Rab25
  • アプリケーション適用あり: ELISA, WB, IHC-P, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant fragment from Rab25, expressed in E. coli.

  • ポジティブ・コントロール
    • WB: MCF7, T47D and GC7901 cell lysates IHC-P: Human esophagus and lung cancer tissues ICC/IF/Flow cytometry: NIH 3T3 cells

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab106175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/10000.
WB 1/500 - 1/2000. Predicted molecular weight: 23 kDa.
IHC-P 1/200 - 1/1000.
ICC/IF 1/200 - 1/1000.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Involved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia (PubMed:17925226). Involved in the regulation of epithelial morphogenesis through the control of CLDN4 expression and localization at tight junctions (By similarity). May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis.
  • 組織特異性Expressed in ovarian epithelium (NOE) and breast tissue. Expressed in ovarian cancer; expression is increased relative to NOE cells. Expression in ovarian cancer is stage dependent, with stage III and stage IV showing higher levels than early stage cancers. Expressed in breast cancer; expression is increased relative to normal breast tissue.
  • 配列類似性Belongs to the small GTPase superfamily. Rab family.
  • 細胞内局在Cell membrane. Cell projection, pseudopodium membrane. Cytoplasmic vesicle. Colocalizes with integrin alpha-V/beta-1 in vesicles at the pseudopodial tips.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CATX 8 antibody
    • CATX-8 antibody
    • CATX8 antibody
    • RAB11C antibody
    • RAB25 antibody
    • RAB25 member RAS oncogene family antibody
    • RAB25_HUMAN antibody
    • Ras related protein Rab 25 antibody
    • Ras-related protein Rab-25 antibody
    • RP11-336K24.10-001 antibody
    see all

Anti-Rab25 antibody [3F12] 画像

  • All lanes : Anti-Rab25 antibody [3F12] (ab106175) at 1/500 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : T47D cell lysate
    Lane 3 : GC7901 cell lysate


    Predicted band size : 23 kDa
  • Immunohistochemical analysis of Rab25 in paraffin embedded esophagus tissue, using ab106175 at a 1/200 dilution.
  • Immunohistochemical analysis of Rab25 in paraffin embedded Human lung cancer tissue, using ab106175 at a 1/200 dilution.
  • Flow cytometric analysis of NIH 3T3 cells using ab106175 (green) and a negative control (purple).

Anti-Rab25 antibody [3F12] (ab106175) 使用論文

ab106175 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"