製品の概要

  • 製品名Anti-RAB21 antibody
    RAB21 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RAB21
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human RAB21.

    (Peptide available as ab89377.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human liver tissue lysate and in the following whole cell lysates: HepG2; HeLa; A431; Jurkat.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab88762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Regulates integrin internalization and recycling, but does not influence the traffic of endosomally translocated receptors in general. As a result, may regulate cell adhesion and migration (By similarity). During the mitosis of adherent cells, controls the endosomal trafficking of integrins which is required for the successful completion of cytokinesis.
    • 組織特異性Widely expressed. In jejunal tissue, predominantly expressed in the apical region of the epithelial cell layer of the villi, weak expression, if any, in the crypt epithelium. Capillary endothelium and some cell types in the lamina propria also show expression.
    • 配列類似性Belongs to the small GTPase superfamily. Rab family.
    • 細胞内局在Endoplasmic reticulum membrane. Golgi apparatus membrane. Early endosome membrane. Cytoplasmic vesicle membrane. Cleavage furrow. In nonpolarized epithelial Caco-2 cells, found in the endoplasmic reticulum; in polarized cells, observed in vesicles in the apical cytoplasm. During mitosis, in mid-telophase, localized in the ingressing cleavage furrow. In late telophase, detected at the opposite poles of the daughter cells, in vesicles at the base of lamellipodia formed by the separating daughter cells.
    • Information by UniProt
    • 参照データベース
    • 別名
      • KIAA0118 antibody
      • RAB 21 antibody
      • RAB21 antibody
      • RAB21, member RAS oncogene family antibody
      • RAB21_HUMAN antibody
      • Ras-related protein Rab-21 antibody
      see all

    Anti-RAB21 antibody 画像

    • All lanes : Anti-RAB21 antibody (ab88762) at 1 µg/ml

      Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 5 : Human liver tissue lysate - total protein (ab29889)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 24 kDa
      Observed band size : 26 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 12.5 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 4 minutes

    Anti-RAB21 antibody (ab88762) 使用論文

    ab88762 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HAEC-human arterial endothelial cells)
    Loading amount 30 µg
    Specification HAEC-human arterial endothelial cells
    Gel Running Conditions Reduced Denaturing (4-15% gradient)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 45°C
    Username

    Mrs. Elena Astanina

    Verified customer

    投稿 May 12 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"