製品の概要

  • 製品名Anti-RAB1B antibody
    RAB1B 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to RAB1B
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 91 - 202 of human RAB1B protein (NP_112243) with a tag

  • ポジティブ・コントロール
    • A-431 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
  • 追加情報ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 22 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Protein transport. Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments.
    • 配列類似性Belongs to the small GTPase superfamily. Rab family.
    • 翻訳後修飾Prenylated; by GGTase II, only after interaction of the substrate with Rab escort protein 1 (REP1).
      AMPylation at Tyr-77 occurs in the switch 2 region and leads to moderate inactivation of the GTPase activity. It appears to prolong the lifetime of the GTP state of RAB1B by restricting access of GTPase effectors to switch 2 and blocking effector-stimulated GTP hydrolysis, thereby rendering RAB1B constitutively active.
    • 細胞内局在Membrane. Cytoplasm. Targeted by REP1 to membranes of specific subcellular compartments including endoplasmic reticulum, Golgi apparatus, and intermediate vesicles between these two compartments. In the GDP-form, colocalizes with GDI in the cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • RAB1B antibody
      • RAB1B member RAS oncogene family antibody
      • RAB1B_HUMAN antibody
      • Ras related protein Rab1B antibody
      • Ras-related protein Rab-1B antibody
      • Small GTP binding protein antibody
      see all

    Anti-RAB1B antibody 画像

    • Anti-RAB1B antibody (ab76721) at 5 µg/ml + immunogen at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

      Predicted band size : 22 kDa
      Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    • Anti-RAB1B antibody (ab76721) at 5 µg/ml + A431 cell lysate at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 22 kDa
      Observed band size : 22 kDa

    Anti-RAB1B antibody (ab76721) 使用論文

    ab76721 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab76721.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"