製品の概要

  • 製品名Anti-RAB1B antibody
    RAB1B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RAB1B
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 165-194 of Human RAB1B (NP_112243.1).

  • ポジティブ・コントロール
    • 293 cell line lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab116336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 22 kDa.

ターゲット情報

  • 機能Protein transport. Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments.
  • 配列類似性Belongs to the small GTPase superfamily. Rab family.
  • 翻訳後修飾Prenylated; by GGTase II, only after interaction of the substrate with Rab escort protein 1 (REP1).
    AMPylation at Tyr-77 occurs in the switch 2 region and leads to moderate inactivation of the GTPase activity. It appears to prolong the lifetime of the GTP state of RAB1B by restricting access of GTPase effectors to switch 2 and blocking effector-stimulated GTP hydrolysis, thereby rendering RAB1B constitutively active.
  • 細胞内局在Membrane. Cytoplasm. Targeted by REP1 to membranes of specific subcellular compartments including endoplasmic reticulum, Golgi apparatus, and intermediate vesicles between these two compartments. In the GDP-form, colocalizes with GDI in the cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • RAB1B antibody
    • RAB1B member RAS oncogene family antibody
    • RAB1B_HUMAN antibody
    • Ras related protein Rab1B antibody
    • Ras-related protein Rab-1B antibody
    • Small GTP binding protein antibody
    see all

Anti-RAB1B antibody 画像

  • Anti-RAB1B antibody (ab116336) at 1/100 dilution + 293 cell line lysate at 35 µg

    Predicted band size : 22 kDa

Anti-RAB1B antibody (ab116336) 使用論文

ab116336 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Sample Mouse Cell (RAW264.7 Murine Macrophage Cell)
Specification RAW264.7 Murine Macrophage Cell
Permeabilization Yes - 0.5% Tween 20 for 1hr @ Roomtemp
Fixative Paraformaldehyde 2.5% pH 7.4
Username

Mr. Michael Connor

Verified customer

投稿 Sep 18 2014

Application Western blot
Loading amount 1e+006 cells
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris, MOPS running buffer)
Sample Human Cell lysate - whole cell (293T)
Specification 293T
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Constantin Takacs

Verified customer

投稿 Nov 19 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"