製品の概要

  • 製品名Anti-Rab1A antibody
    Rab1A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Rab1A
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal amino acids 155 - 204 (AKNATNVEQS FMTMAAEIKK RMGPGATAGG AEKSNVKIQS TPVKQSGGGC) of Human Rab1A (NP_004152).

  • ポジティブ・コントロール
    • Fetal muscle lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab97956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 23 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/100.

ターゲット情報

  • 機能The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB1A regulates vesicular protein transport from the endoplasmic reticulum (ER) to the Golgi compartment and on to the cell surface, and plays a role in IL-8 and growth hormone secretion. Regulates the level of CASR present at the cell membrane. Plays a role in cell adhesion and cell migration, via its role in protein trafficking. Plays a role in autophagosome assembly and cellular defense reactions against pathogenic bacteria. Plays a role in microtubule-dependent protein transport by early endosomes and in anterograde melanosome transport.
  • 配列類似性Belongs to the small GTPase superfamily. Rab family.
  • 翻訳後修飾Phosphorylated by CDK1 kinase during mitosis.
    Phosphocholinated at Ser-79 by L.pneumophila AnkX, leading to displace GDP dissociation inhibitors (GDI). Both GDP-bound and GTP-bound forms can be phosphocholinated. Dephosphocholinated by L.pneumophila Lem3, restoring accessibility to L.pneumophila GTPase effector LepB.
  • 細胞内局在Golgi apparatus. Endoplasmic reticulum. Early endosome. Cytoplasm, cytosol. Membrane. Melanosome. Alternates between membrane-associated and cytosolic forms.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GTP binding protein RAB 1A antibody
    • mKIAA3012 antibody
    • RAB 1 antibody
    • Rab 1A antibody
    • RAB1 antibody
    • RAB1, member RAS oncogene family antibody
    • Rab1A antibody
    • RAB1A member RAS oncogene family antibody
    • RAB1A_HUMAN antibody
    • Ras related protein Rab 1A antibody
    • Ras-associated protein RAB1 antibody
    • Ras-related protein Rab-1A antibody
    • YPT1 antibody
    • YPT1 related protein antibody
    • YPT1-related protein antibody
    see all

Anti-Rab1A antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling Rab1A with ab97956 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - Rab1A. Right - Merge.
  • Anti-Rab1A antibody (ab97956) at 1/2000 dilution + Human Panc-1 whole cell lysate at 35 µg

    Secondary
    HRP-conjugated Donkey anti-rabbit IgG polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an Abreview submitted by Armen Petrosyan

    See Abreview

  • All lanes : Anti-Rab1A antibody (ab97956) at 2 µg/ml

    Lane 1 : HepG2 cell lysate
    Lane 2 : HepG2 cell lysate with blocking peptide at 5 µg/ml

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 23 kDa
  • Anti-Rab1A antibody (ab97956) at 1 µg/ml + Human fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 23 kDa
    Gel concentration: 12%

Anti-Rab1A antibody (ab97956) 使用論文

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 35 µg
Gel Running Conditions Reduced Denaturing (15% SDS-PAGE)
Sample Human Cell lysate - whole cell (Panc-1 cells)
Specification Panc-1 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

投稿 Nov 13 2013

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample Human Cell (LNCaPcells)
Specification LNCaPcells
Permeabilization No
Fixative Paraformaldehyde
Username

Dr. Armen Petrosyan

Verified customer

投稿 Nov 13 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"