製品の概要

  • 製品名Anti-Rab11A antibody
    Rab11A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Rab11A
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Sheep, Chicken, Cow, Rhesus monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Rab11.

    (Peptide available as ab66706.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following Human Whole Cell Lysates: HeLa, Jurkat, MCF7, SHSY-5Y

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab65200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能Regulates endocytic recycling. May exert its functions by interacting with multiple effector proteins in different complexes. Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis (By similarity). Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes.
  • 配列類似性Belongs to the small GTPase superfamily. Rab family.
  • 細胞内局在Cell membrane. Recycling endosome membrane. Cleavage furrow. Translocates with RAB11FIP2 from the vesicles of the endocytic recycling compartment (ERC) to the plasma membrane. Localizes to the cleavage furrow. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB8A in apical membrane initiation sites (AMIS) during the generation of apical surface and luminogenesis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • MGC1490 antibody
    • Rab 11 antibody
    • Rab 11A antibody
    • RAB 11A, member oncogene family antibody
    • Rab-11 antibody
    • RAB11 A antibody
    • RAB11 antibody
    • Rab11a antibody
    • RAB11A member RAS oncogene family antibody
    • Ras related protein Rab 11A antibody
    • Ras related protein Rab11A antibody
    • Ras-related protein Rab-11A antibody
    • RB11A_HUMAN antibody
    • YL 8 antibody
    • YL8 antibody
    see all

Anti-Rab11A antibody 画像

  • ICC/IF image of ab65200 stained MCF-7 cells. The cells were fixed with 4% PFA (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65200 at 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 100% methanol (5 min) fixed MCF7 cells at 5µg/ml.
  • All lanes : Anti-Rab11A antibody (ab65200) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 24 kDa
    Observed band size : 24 kDa
    Additional bands at : 26 kDa (possible post-translational modification),45 kDa,72 kDa. We are unsure as to the identity of these extra bands.

Anti-Rab11A antibody (ab65200) 使用論文

This product has been referenced in:
  • Magnusson K  et al. An imidazole functionalized pentameric thiophene displays different staining patterns in normal and malignant cells. Front Chem 3:58 (2015). ICC/IF ; Human . Read more (PubMed: 26501054) »
  • Chen D  et al. Glioma cell proliferation controlled by ERK activity-dependent surface expression of PDGFRA. PLoS One 9:e87281 (2014). ICC/IF ; Human . Read more (PubMed: 24489888) »

See all 5 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Monkey Cell (COS7)
Permeabilization Yes - 0.2%Triton
Specification COS7
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative Formaldehyde
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Abcam user community

Verified customer

投稿 Oct 07 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MCF7)
Loading amount 10 µg
Specification MCF7
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Abcam user community

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投稿 Oct 27 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (thoracic-lumbar spinal cord)
Loading amount 1 µg
Specification thoracic-lumbar spinal cord
Gel Running Conditions Reduced Denaturing (Invitrogen 4-12% NP0323)
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: RT°C
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Dr. Thomas Sardella

Verified customer

投稿 Feb 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (A431 Cells)
Loading amount 5 µg
Specification A431 Cells
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

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投稿 Jun 15 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Fibrosarcoma)
Specification Fibrosarcoma
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100
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投稿 Jun 04 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"