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Our Abpromise guarantee covers the use of ab3612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 18384686|
|WB||Use at an assay dependent concentration. Detects a band of approximately 22 kDa (predicted molecular weight: 24 kDa).Can be blocked with Human Rab11 peptide (ab41779). By Western blot, this antibody detects an ~22 kDa protein representing Rab 11 in CV1, PC3, and HeLa cell extract. Another less prominent, unknown band is also detected by this antibody at ~35 kDa.|
|IP||Use at an assay dependent concentration. PubMed: 17686995|
Immunofluorescence analysis of HeLa cells, staining Rab11 (red) with ab3612.
Cells were fixed with paraformaldehyde for 10 minutes, blocked in PBS with 5% normal goat serum and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/200). An AlexaFluor®594-conjugated anti-rabbit IgG (1/1000) was used as the secondary antibody.
ab3612 staining HEK 293 cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.012% saponin prior to blocking in 1% BSA for 30 minutes at 37°C. The primary antibody was diluted 1/200 and incubated with the sample for 2 hours at 37°C. A biotinylated goat anti-rabbit antibody, diluted 1/200, was used as the secondary.
This image depicts ab3612 recognizing endogenous Rab11 (red). Rab11 was used as a recycling endosome marker.
ab3612 used on PC3 cell extract.
ab3612 staining Rab11 in Canine kidney cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using 0.5% saponin, then incubated with ab3612 at a 1/200 dilution for 1.5 hours at 20°C. The secondary used was an Alexa-Fluor 568 conjugated donkey anti-rabbit polyclonal, used at a 1/350 dilution.
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