製品の概要

  • 製品名Anti-RAB10 antibody [4E2]
    RAB10 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [4E2] to RAB10
  • アプリケーション適用あり: ICC/IF, WB, ELISA, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Purified recombinant fragment of Human RAB10 expressed in E. coli.

  • ポジティブ・コントロール
    • Hela and NIH/3T3 cell lysates; LOVO cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab104859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/200 - 1/1000.
WB 1/500 - 1/2000. Predicted molecular weight: 23 kDa.
ELISA 1/10000.
Flow Cyt 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). That Rab is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane. Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane. In parallel, it regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response. Plays also a specific role in asymmetric protein transport to the plasma membrane within the polarized neuron and epithelial cells. In neurons, it is involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane while in epithelial cells, it regulates transport from the Golgi to the basolateral membrane. Moreover, may play a role in the basolateral recycling pathway and in phagosome maturation. According to PubMed:23263280, may play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion.
  • 配列類似性Belongs to the small GTPase superfamily. Rab family.
  • 細胞内局在Cytoplasmic vesicle membrane. Golgi apparatus membrane. Golgi apparatus, trans-Golgi network membrane. Endosome membrane. Recycling endosome membrane. Cytoplasmic vesicle, phagosome membrane. Cell projection, cilium. Endoplasmic reticulum membrane. Associates with SLC2A4/GLUT4 storage vesicles (PubMed:22908308). Localizes to the base of the cilium (PubMed:20576682). Transiently associates with phagosomes (By similarity). Localizes to the endoplasmic reticulum at domains of new tubule growth (PubMed:23263280).
  • Information by UniProt
  • 参照データベース
  • 別名
    • GTP binding protein RAB10 antibody
    • Rab10 antibody
    • RAB10 member RAS oncogene family antibody
    • RAB10_HUMAN antibody
    • Ras related GTP binding protein antibody
    • Ras related GTP binding protein RAB10 antibody
    • Ras-related protein Rab-10 antibody
    see all

Anti-RAB10 antibody [4E2] 画像

  • All lanes : Anti-RAB10 antibody [4E2] (ab104859) at 1/500 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : NIH/3T3 cell lysate


    Predicted band size : 23 kDa
  • Immunofluorescence analysis of LOVO cells using ab104859 (green). Blue: DRAQ5 fluorescent DNA dye.
  • Overlay histogram showing HeLa cells stained with ab104859 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104859, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91653, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-RAB10 antibody [4E2] (ab104859) 使用論文

This product has been referenced in:
  • Lindsay AJ  et al. Identification and characterization of multiple novel Rab-myosin Va interactions. Mol Biol Cell 24:3420-34 (2013). ICC/IF . Read more (PubMed: 24006491) »
  • Paul C & Robaire B Impaired function of the blood-testis barrier during aging is preceded by a decline in cell adhesion proteins and GTPases. PLoS One 8:e84354 (2013). WB ; Rat . Read more (PubMed: 24391944) »

See all 2 Publications for this product

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I am very pleased to hear you would like to accept our offer and test ab104859 in Drosophila. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please sub...

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Thank you very much for your interest in ab104859.

I havetalked to the lab and it look as though the immunogen for ab104859 is in an area tha sharesa high level of homology with Drosophila(>85%).

Therefore, I can offer a discount o...

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Thank you for your inquiry. Based on the BLAST results, the immunogen sequence used to generate ab104859 is only 69% homologous with drosophila. This is a bit low, so cross-reactivity with drosophila would probably not be expected with this ant...

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