製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to R2D2
  • 特異性
    The antibody specifically recognises the immunising peptide in a blocking western blot. All four observed bands are blocked, showing that they are specific. There is also low background and high titre in ELISA. Please let us know if you can explain these bands.
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Drosophila melanogaster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Fruit fly (Drosophila melanogaster) R2D2.

    (Peptide available as ab17670.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Drosophila lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab27650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).

ターゲット情報

  • 関連性
    The RNA interference (RNAi) pathway is initiated by processing long double-stranded RNA into small interfering RNA (siRNA). The siRNA-generating enzyme consists of DCR-2 and R2D2. R2D2 is homologous to the Caenorhabditis elegans RNAi protein RDE-4.
  • 細胞内局在
    Nuclear
  • 参照データベース
    • 別名
      • CG7138 antibody
      • CG7138 PA antibody
      • LD06392p antibody

    画像

    • All lanes : Anti-R2D2 antibody (ab27650) at 1 µg/ml

      Lane 1 : Drosophila lysate
      Lane 2 : Drosophila lysate with Human R2D2 peptide (ab17670) at 1 µg/ml

      Lysates/proteins at 20 µg per lane.


      Predicted band size : 35 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 39 kDa (possible cross reactivity),55 kDa (possible cross reactivity),85 kDa (possible cross reactivity).

    プロトコール

    参考文献

    ab27650 has not yet been referenced specifically in any publications.

    レビューと Q&A

    There are currently no Customer reviews or Questions for ab27650.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録