製品の概要
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製品名Anti-R Phycoerythrin antibody (Biotin)
R Phycoerythrin 一次抗体 製品一覧 -
製品の詳細Goat polyclonal to R Phycoerythrin (Biotin)
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由来種Goat
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標識Biotin
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特異性This antibody will cross react with B Phycoerythrin. Reactivity with other phycobiliproteins is unknown.
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アプリケーション適用あり: Dot blot, WB, ELISAmore details
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免疫原
Full length protein Highly purified R Phycoerythrin from the seaweed gracilaria.
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特記事項
10-20 Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) molecules per goat IgG molecule.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.01% Sodium Azide
Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
IgG and Protease free. -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）This antibody was prepared from monospecific antiserum by immunoaffinity chromatography using R Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab34730 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Dot blot | Use at an assay dependent dilution. | |
| WB | 1/1000 - 1/3000. Detects a band of approximately 240 kDa. | |
| ELISA | 1/8000 - 1/32000. |
ターゲット情報
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関連性Phycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.
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細胞内局在Plastid; chloroplast; chloroplast thylakoid lumen. Periphery of the rods of the phycobilisome.
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別名
- R phycoerythrin alpha subunit antibody
- R phycoerythrin beta subunit antibody
- rpeA antibody
- rpeB antibody
プロトコール
参考文献
ab34730 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"