Anti-R Phycoerythrin 抗体 (Alkaline Phosphatase) (ab34729)

製品の概要

  • 製品名
    Anti-R Phycoerythrin antibody (Alkaline Phosphatase)
    R Phycoerythrin 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to R Phycoerythrin (Alkaline Phosphatase)
  • 標識
    Alkaline Phosphatase
  • アプリケーション
    適用あり: Dot blot, ELISA, WBmore details
  • 免疫原

    R-Phycoerythrin from the seaweed gracilaria.

  • 特記事項

    Alkaline Phosphatase (from Calf Intestine, Molecular Weight 140 kDa). Conjugation Reference: Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.01% Sodium Azide
    Constituents: 50% Glycerol, 10mg/ml BSA, 0.15M Sodium chloride, 0.05M Tris HCl, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified by immunoaffinity chromatography using a R-Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab34729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Dot blot
ELISA
WB
  • 追加情報
    ELISA: 1/1,000 - 1/5,000. Assayed against 1.0 ug of R-Phycoerythrin in a standard
    capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 240 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性
      Phycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.
    • 細胞内局在
      Plastid; chloroplast; chloroplast thylakoid lumen. Periphery of the rods of the phycobilisome.
    • 別名
      • R phycoerythrin alpha subunit antibody
      • R phycoerythrin beta subunit antibody
      • rpeA antibody
      • rpeB antibody

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    参考文献

    ab34729 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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