製品の概要

  • 製品名Quick Cell Proliferation Assay Kit
    Cell proliferation キット 製品一覧
  • サンプルの種類
    Adherent cells, Suspension cells
  • アッセイタイプQuantitative
  • 全工程の試験時間
    4h 00m
  • 製品の概要

    Quick Cell Proliferation Assay Kit (ab65473) provides all reagents and detailed instructions for a fast and sensitive quantification of cell proliferation and viability. The assay is based on the cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases. Expansion in the number of viable cells resulted in an increase in the activity of the mitochondrial dehydrogenases, which leads to the increase in the amount of formazan dye formed. The formazan dye produced by viable cells can be quantified by multi-well spectrophotometer (microtiter plate reader) by measuring the absorbance of the dye solution at 440 nm. The assay can be used for the measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients, etc. It can also be used for the analysis of cytotoxic compounds like anticancer drugs and many other toxic agents and pharmaceutical compounds. The new method is so simple, requiring no washing, no harvesting, and no solubilization steps, and is faster and more sensitive than MTT, XTT, or MTS-based assays. The entire assay can be performed in a microtiter plate.
    Visit our FAQs page for tips and troubleshooting.

  • アプリケーション適用あり: Functional Studiesmore details

製品の特性

  • 関連性Cell proliferation is the multiplication or reproduction of cells, as a result of cell growth and cell division, resulting in the expansion of a cell population.

アプリケーション

Our Abpromise guarantee covers the use of ab65473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

プロトコール

Quick Cell Proliferation Assay Kit (ab65473) 使用論文

This product has been referenced in:
  • Guo S  et al. Lipopolysaccharide Causes an Increase in Intestinal Tight Junction Permeability in Vitro and in Vivo by Inducing Enterocyte Membrane Expression and Localization of TLR-4 and CD14. Am J Pathol 182:375-87 (2013). Read more (PubMed: 23201091) »
  • Shanks K  et al. Neuroimmune semaphorin 4D is necessary for optimal lung allergic inflammation. Mol Immunol 56:480-7 (2013). Read more (PubMed: 23911404) »

See all 5 Publications for this product

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The absorption measured at the reference wavelength value is subtracted from each reading. This is to normalize the readings against a non changing value. One wavelength reports the change in a...

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The assay kit has not been designed to use with a standard curve as such. You can indeed reference your readings if you need to. Any cell type that is actively dividing in culture can be used as a positive control. Any cell type that is treated with a...

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The primary difference between the quick cell proliferiation assay (ab65473) and quick cell plus proliferation assay (ab65475) is that the ab65475 is more sensitive and more stable.

Thank you for contacting us and your interest in our products.

The Quick Cell Proliferation Assay Kit (ab65473) and Quick Cell Proliferation Assay Kit II (ab65475) should not be affected by serum in your culture medium. Any differences from th...

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Thank you for your inquiry. I have asked the laboratory with your question. Indeed, the laboratory recommend just that if the cells are cultured in different volume of culture medium, increase or decrease the amount of WST-1/ECS solution correspond...

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ab65474 - enables to perform 2500 assays instead of 500 (like ab65473) and includes a premade stop solution (unlike ab65473). Also the kits include different tetrazolium salts (WST or WST-1 reagent). More details cannot be given out due to proprietary...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"