製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to QKI
  • 特異性
    ab77176 is expected to recognise all reported isoforms of QKI.
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Dog, Human, Pig, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to Human QKI aa 6-17 (N terminal). NP_006766.1; NP_996735.1; NP_996736.1; NP_996737.1
    Sequence:

    ETKEKPKPTPDY-C

  • ポジティブ・コントロール
    • Mouse brain lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 38 kDa.
ELISA 1/64000.

Peptide ELISA

ターゲット情報

  • 機能
    RNA-binding protein that plays a central role in myelinization (PubMed:16641098). Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Regulates target mRNA stability (PubMed:23630077). In addition, acts by regulating pre-mRNA splicing, mRNA export and protein translation. Required to protect and promote stability of mRNAs such as MBP and CDKN1B. Regulator of oligodendrocyte differentiation and maturation in the brain that may play a role in myelin and oligodendrocyte dysfunction in schizophrenia (PubMed:16641098). Participates in mRNA transport by regulating the nuclear export of MBP mRNA. Also involved in regulation of mRNA splicing of MAG pre-mRNA. Acts as a translational repressor.
  • 組織特異性
    Expressed in the frontal cortex of brain. Down-regulated in the brain of schizophrenic patients.
  • 配列類似性
    Contains 1 KH domain.
  • ドメイン
    The KH domain and the Qua2 region are involved in RNA binding.
  • 翻訳後修飾
    Methylated by PRMT1.
    Tyrosine phosphorylated at its C-terminus, probably by FYN. Phosphorylation leads to decreased mRNA-binding affinity, affecting transport and/or stabilization of MBP mRNA.
  • 細胞内局在
    Nucleus. Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp586I0923 antibody
    • HKQ antibody
    • Homolog of mouse quaking QKI KH domain RNA binding protein antibody
    • Hqk antibody
    • HQK1 antibody
    • HqkI antibody
    • OTTHUMP00000017581 antibody
    • OTTHUMP00000017582 antibody
    • OTTHUMP00000017583 antibody
    • Protein quaking antibody
    • QK antibody
    • QK1 antibody
    • QK3 antibody
    • QKI antibody
    • QKI_HUMAN antibody
    • QKI1 antibody
    • Quaking homolog antibody
    • Quaking homolog KH domain RNA binding antibody
    • Quaking homolog KH domain RNA binding mouse antibody
    • Quaking isoform 1 antibody
    • Quaking protein antibody
    • RNA binding protein HQK antibody
    see all

画像

  • Anti-QKI antibody (ab77176) at 0.3 µg/ml + mouse brain lysate in RIPA buffer at 35 µg

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
    Primary incubation was 1 hour. Detected by chemiluminescence.

プロトコール

参考文献

ab77176 has not yet been referenced specifically in any publications.

レビューと Q&A

There are currently no Customer reviews or Questions for ab77176.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録